Many factors have guided our plate decisions and these have not been taken lightly. Principally we recommend users to use the three plate makes and models described below. The plates are used for fluorescence, luminescence and microscopy. The assay development plate, subset libraries and library are provided in the correct plate type and you do not have to purchase these separately. Users only need to purchase these plates for assay building prior to the assay development plate step and post-screen validation for the verification of hits.
Perkin Elmer clear bottomed plates for all high content microscopy experiments (View plate) (#60017460). A plastic coverslip – take care not to touch or scratch this when manipulating the plates prior to acquisition. The coverslip is attached to the base of the plate, has been treated to be cell culture ready and is 170mm thick. Coverslip thickness is important when considering objectives and acquisition modes. To uncomplicated things we have standardized the use of the 170mm coverslip.
Greiner Bio One Ltd (#781073) 384 plate, cell culture ready. White and solid, do not leave in view of direct sunlight, as the plates will begin to luminesce, leading to background affects.
Matrix black, cell culture ready plates (#4318) for all fluorescent plate reader assays.
As you will see, all plates have been treated for adherent-based cell growth and Kc167 and S2R+ Drosophila cell lines all adhere well to the bottom of each of these plate types. The cells will de-adhere if grown in excess of confluence.