Our service supplies to the wider scientific community whole genome screens at low cost. We offer either pre-aliquoted whole genome RNAi libraries or sub-libraries of target probes.
We support both plate reader assays, high-content microscopy as well as the equipment needed to process these samples in a high-throughput fashion. This can either be used to identify genes involved in disease representing future drug targets, or to identify genes involved in biological processes.
The major role of our staff is to generate the libraries and to assist screeners through the process before they carry out their experiments at the facility.
The screening process can be split into a number of discrete steps and organised in two stages between your institute and the SRSF.
Assays are usually developed in the screeners laboratory before discussion with our staff and then followed by the running of an assay development plate.
We provide expert help and assistance, including assay development and optimization, data and image analysis, and planning of follow-up assays and secondary hit determination.
In the first instance, contact us to discuss your screening requirements and we will advise you on the options available.
We offer genome-wide RNAi screens, a flexible service, including bespoke library construction and use of our equipment.
Once your requirements have been discussed, the screening process requires a number of forms to be completed before work can commence. When the assay has been developed you will be placed in queue ready to screen.
We thank the MRC, through funding the CDGB, for jointly funding some small equipment located in the SRSF.
We will be running a workshop on the High Content Screening of Stem Cells in April 2014. More details to follow.
The SRSF now offers genome-wide siRNA screening using the Dharmacon On-Target Plus siRNA Human Genome collection. Latest: Human siRNA screening fees from November 2012. Read more.
Fisher KH, Brown S, Zeidler MP.
Designing RNAi Screens to Identify JAK/STAT Pathway Components.
Methods Mol Biol. 2013;967:81-97. In press.
Brown S. (2012)
"Putting the "Fun" into functional genomics. A review of RNAi genome-wide cellular screens."
European Pharmaceutical Reviews 18 Dec; 17(6):48-50
Drosophila cells are the ideal tool for genome-wide screens because they represent a low complexity, low cost system amenable to RNAi screening.
Please feel free to contact us about our screening service. We are open to applications from both UK and overseas research institutes.